Amplicon-based NGS Panel: Defining Precision in Targeted Sequencing

Advancements in genomic research demand tools that combine accuracy, scalability, and flexibility. The Amplicon-based NGS Panel offers researchers and clinical labs a targeted sequencing approach that delivers high sensitivity and specificity across a variety of applications. This method focuses on selectively amplifying genomic regions of interest, ensuring that even low frequency variants are detected reliably. Its efficiency and precision make it a cornerstone in modern genomics.

Customizable Targeting for Specific Research Needs

One of the primary strengths of an amplicon based NGS panel is its adaptability. Customizable primer designs allow laboratories to focus on genes, exons, or hotspots that are most relevant to their research or clinical testing goals. This precision reduces unnecessary sequencing overhead while maximizing actionable data. Scientists can design panels to match the exact scope of their studies, whether exploring inherited diseases, cancer mutations, or pathogen genomics.

The flexibility of amplicon-based panels also supports iterative research. Panels can be expanded or refined over time as new targets emerge, enabling long term projects to adapt without starting from scratch. This modular approach is especially valuable in fields such as infectious disease surveillance and oncology, where emerging variants or mutations require rapid testing solutions.

Key Advantages of Amplicon-Based Panels

• High sensitivity for rare variants: Detecting mutations present in as little as 1% of a sample is possible, making it ideal for oncology and infectious disease research.
• Efficient use of sequencing resources: Targeted amplification concentrates sequencing depth on critical regions, lowering costs and reducing data processing time.
• Versatile sample compatibility: Panels can accommodate DNA or RNA from diverse sources, including FFPE samples, blood, saliva, or even environmental samples for public health studies.
• Rapid assay development: Patented primer design algorithms enable the creation of custom panels with reduced optimization time.
• Scalable multiplexing: Multiple samples or targets can be processed simultaneously, increasing throughput without compromising accuracy.

Applications in Clinical and Public Health Labs

Amplicon based panels play a key role in clinical and public health laboratories. They support genetic screening, biomarker discovery, and pathogen detection, providing timely and actionable insights. These panels also contribute to drug development and biopharma research by identifying mutations and gene expression profiles that guide therapeutic decisions and personalized medicine programs.

Enhanced Accuracy with Molecular Identifiers

Modern amplicon sequencing platforms often incorporate unique molecular identifiers (UMIs), which help minimize amplification bias and sequencing errors. This ensures that variant frequencies are represented accurately, a crucial factor for detecting low frequency mutations in heterogeneous samples. UMIs also enhance reproducibility across experiments, providing confidence in both research and clinical outcomes.

Partnering for Custom Solutions

Working with experts in amplicon-based NGS panel design can streamline workflows and accelerate results. Specialized teams create tailored panels, provide validation support, and offer guidance on best practices to ensure reliable and compliant outcomes. These solutions help researchers and labs enhance data accuracy while focusing on critical genomic regions.